PROTSMART™ Plasma Ultrafiltrator
First-class concentrator of proteins and platelets
Platelets and mononucleated cells have numerous advantages as biological products for the treatment of many pathologies.
They are easily prepared autologous products, cause minimal complications, and have a broad range of potential actions.
Platelets, mononucleated cells and the liquid portion (plasma) of PRP simultaneously release many proteins and factors with different anabolic functions that reduce inflammation and promote angiogenesis and tissue regeneration. Wound healing in response to injury involves the coordination of a large number of complex cellular and molecular events within the body.
This response is defined by the need for cells to travel to the site of injury, proliferate, form blood vessels, and, eventually, extracellular matrix (ECM) to restore both the structure and the function of the damaged tissue.
The aqueous part of PRP is plasma, which is an acellular component containing proteins that are important for tissue regeneration.
These proteins are involved in the tissue healing process and enable anti-inflammatory effects, clot stabilization, anti-microbial actions, cell-matrix adhesion, and tissue synthesis while acting overall as a ubiquitous broad-spectrum proteinase inhibitor.
These cellular processes are driven by a wide variety of proteins, growth factors, and cytokines that act to control cell functions.
One of the most important plasma cytokines is a2M.
The alpha-2-macroglobulin molecule is synthesized mainly in the liver, but also locally by macrophages, fibroblasts, and it is the largest major nonimmunoglobulin protein in plasma. Alpha 2 macroglobulin acts as an antiprotease and can inactivate an enormous variety of proteinases.
It works as an inhibitor of coagulation by inhibiting thrombin. Alpha-2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β.
The α2M offers great potential for the regulation of cytokine homeostasis in blood and tissue, a critical point in the pathogenesis of several diseases.
Mechanical concentration accessory,
compatible with 20 ml syringes.
α2M is known as an ubiquitous broad-spectrum proteinase inhibitor.
α2M traps the proteinases released by granu- locytes during inflammation, such as matrix metalloproteinases (MMPs).
α2M is a master inhibitor of many types of cartilage- degrading enzymes, is an exciting and potential candidate for OA treatment.
α2M may play an important role in regulating inflammatory reactions in joint diseases, not only because of its action as an inhibitor of proteases, but also because of its potential to modulate several cell types involved in joint inflammation.
α2M is also involved in the immune defense reactions
with a role of protection against toxic effects by an over production of pro-inflammatory cytokines during inflammation.
But the plasma protease inhibitor a2M is not present in sufficient concentrations to inactivate the high levels of catabolic factors found in OA synovial fluid. Findings suggest that supplemental intra-articular A2M
provides chondral protection in post-traumatic OA.
CONCENTRATION OF A2M AND ANABOLIC MOLECULES
ELIMINATION OF ALL CELL MEMBRANES
What is it for?
ProtSmart™ is a class IIa medical device, designed for plasma concentration and blood components. ProtSmart™ is a hollow capillary membrane device that can be used downstream of the blood product preparation method, in order to concentrate more platelets and plasma PRP proteins or even simply the plasma proteins of PPP. The result is a concentration of solutes over a certain molecular weight (greater than the size of the pores) on one side of the membrane, and a loss of liquids and small solutes (smaller than the size of the pores) on the other.
This step is particularly useful if you want to obtain a blood product of high concentrations in small volumes.
In fact, the three ProtSmart™ models available make it possible to obtain autologous preparations of just a few ml, with platelet and protein concentrations that even reach 4-6 times the basal concentrations in whole blood.
ProtSmart™ can concentrate PRP and PPP with a volume reduction of the order of 70-75%.
During this push phase, the pressure gradient created between the two sides of the hollow capillary membrane allows plasma water to escape from its pores.
At the end of the procedure, a simple aspiration enables recovery of the concentrated plasma, from inside the ProtSmart™ device.
This kit is designed to provide at least 6x concentration of A2M, cells and Fibrinogen Proteins.
ProtSmart™ was born from the combination of capillary membrane filtration technologies and the concentration of blood products.
Thanks to a specially developed capillary membrane morphology with an average pore size of 15,000 Da, it is possible to eliminate plasma water thus concentrating the platelets, mononucleated cell, bone marrow and growth factors like alpha 2 macroglobulins present in the plasma.
A2M also binds up pro-inflammatory molecules TNF-a, TGF-b, and IL-1b7.
While IRAP (Interleukin-1 receptor antagonist protein) is present at much higher levels in bone marrow aspirate.
Instructions for use
After checking the product and packaging integrity, proceed as explained below.
The concentration procedure takes place through the use of at least 1 syringe (recommended volume 20 ml) and the mechanical accessory (Fig. A) and the entire procedure is shown in the figure sequence 1-7. Fig A: Front view of the mechanical accessory with the 20 ml syringe already connected (the syringe has to be pre-filled with the fluid to be concentrated: max. loading volume for ProtSmart™ 2 is 8 ml, max loading volume for ProtSmart™ 6 is 16 ml).
Once the syringe is filled with the fluid to be concentrated, it should be connected in sterile mode with the ProtSmart™ device. The system can be then placed in the relevant slot on to the mechanical accessory for concentration.
Turn the knob until it touches the syringe plunger. Fig. B: Rear view of the mechanical accessory for concentration with the syringe of 20 ml (pre-filled) already mounted and connected. It is recommended to check the position of the 3-way stopcock at the bottom; this should be positioned to enable a one-way flow between the syringe and ProtsmartTM device. Once the whole system is assembled, fit the protective cover provided together with the mechanical accessory on the base of the same (the cover is not shown in the pictures below to facilitate understanding of the procedure).
MAXILLOFACIAL AND ODONTOSTOMATIC SURGERY
Bone and soft tissue regeneration
PLASTIC AND AESTHETIC MEDICINE
Anti-aging, bio-revitalization and biological filler
ORTHOPEDICS AND SPORT MEDICINE
Musculo-skeletal iniuries, osteoarthrosis, tendonopathy cartilage regeneration
Acute and chronic ulcers
Scars, alopecia and hair transplantation
GYNECOLOGY AND UROLOGY
Scleroderma, Peyronie’s disease, erectile dysfuncion, tissue rejuvenation
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